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The First Round draw is seeded based on the final league positions of teams in the 2013/14 season, and then split regionally between north and south.
The draw will be announced at 10am on Tuesday 17th June, the full list of seeds and non-seeds can be found below.
North - seeds
1. Barnsley
2. Blackburn Rovers
3. Blackpool
4. Bolton Wanderers
5. Bradford City
6. Derby County
7. Doncaster Rovers
8. Huddersfield Town
9. Leeds United
10. Middlesbrough
11. Nottingham Forest
12. Port Vale
13. Preston North End
14. Rotherham United
15. Sheffield United
16. Sheffield Wednesday
17. Wigan Athletic
North - non-seeds
18. Accrington Stanley
19. Burton Albion
20. Bury
21. Carlisle United
22. Chesterfield
23. Crewe Alexandra
24. Fleetwood Town
25. Hartlepool United
26. Mansfield Town
27. Morecambe
28. Notts County
29. Oldham Athletic
30. Rochdale
31. Scunthorpe United
32. Shrewsbury Town
33. Tranmere Rovers
34. York City
South - seeds
1. AFC Bournemouth
2. Birmingham City
3. Brentford
4. Brighton & Hove Albion
5. Bristol City
6. Cardiff City
7. Charlton Athletic
8. Ipswich Town
9. Leyton Orient
10. Millwall
11. Milton Keynes Dons
12. Peterborough United
13. Reading
14. Swindon Town
15. Walsall
16. Watford
17. Wolverhampton Wanderers
18. Yeovil Town
South - non-seeds
19. AFC Wimbledon
20. Cambridge United
21. Cheltenham Town
22. Colchester United
23. Coventry City
24. Crawley Town
25. Dagenham & Redbridge
26. Exeter City
27. Gillingham
28. Luton Town
29. Newport County
30. Northampton Town
31. Oxford United
32. Plymouth Argyle
33. Portsmouth
34. Southend United
35. Stevenage
36. Wycombe Wanderers